RANKL-binding peptide promotes ectopic bone formation induced by BMP-2 gene transfer in murine gastrocnemius muscle
نویسندگان
چکیده
منابع مشابه
Bone formation induced by BMP-2 in human osteosarcoma cells
Our previous studies demonstrated that BMP-2 inhibits the tumorigenicity of cancer stem cells identified as cells with high aldehyde dehydrogenase activity (ALDH(br) cells) from the human osteosarcoma cell line OS99-1. We further investigated whether BMP-2 is capable of inducing bone formation in OS99-1 cells. Flow cytometry sorting was used to isolate tumorigenic ALDH(br) and non-tumorigenic A...
متن کاملEnhancing Ectopic Bone Formation in Canine Masseter Muscle by Loading Mesenchymal Stem Cells onto Natural Bovine Bone Minerals.
Objectives- To assess the ectopic bone formation in canine masseter muscle following the implantation of the natural bovine bone minerals (NBM) loaded with canine mesenchymal stem cells (MSCs).Design- Experimental study.Animals- four mongrel dogs.Procedures- Tripotent MSCs isolated from the canine bone marrow were loaded onto the NBM sponges and allowed to adhere. The cell-loaded scaffolds were...
متن کاملSimultaneous gene transfer of bone morphogenetic protein (BMP) -2 and BMP-7 by in vivo electroporation induces rapid bone formation and BMP-4 expression
BACKGROUND Transcutaneous in vivo electroporation is expected to be an effective gene-transfer method for promoting bone regeneration using the BMP-2 plasmid vector. To promote enhanced osteoinduction using this method, we simultaneously transferred cDNAs for BMP-2 and BMP-7, as inserts in the non-viral vector pCAGGS. METHODS First, an in vitro study was carried out to confirm the expression ...
متن کاملHeparin potentiates the in vivo ectopic bone formation induced by bone morphogenetic protein-2.
Although bone morphogenetic proteins (BMPs) are clinically useful for bone regeneration, large amounts are required to induce new bone formation in monkeys and humans. We found recently that heparin stimulates BMP activity in vitro (Takada, T., Katagiri, T., Ifuku, M., Morimura, N., Kobayashi, M., Hasegawa, K., Ogamo, A., and Kamijo, R. (2003) J. Biol. Chem. 278, 43229-43235). In the present st...
متن کاملComparison of liposomal formulations incorporating BMP-2 peptide to induce bone tissue engineering
Objective(s): Fabricating a biomimetic scaffold platform combined with controlled release of bioactive agents is a practical approach for bone tissue engineering. Controlled delivery of peptides and growth factors which play a significant role in osteogenesis is an important issue reducing the associated adverse effects and leading to cost-effectiveness. Materials and Methods: We develope...
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ژورنال
عنوان ژورنال: Dental, Oral and Maxillofacial Research
سال: 2020
ISSN: 2633-4291
DOI: 10.15761/domr.1000327